Journal: Oncology Letters

Article Title: The combined use of EphA2/MMP-2 expression and MRI findings contributes to the determination of cerebral glioma grade

doi: 10.3892/ol.2019.10912

Figure Lengend Snippet: Expression of MMP-2 and EphA2 in cerebral glioma samples, according to the pathological grade of the tumor.

Article Snippet: One slide was routinely stained with hematoxylin and eosin in 25°C for 1–5 min. After blocking in BSA solution 37°C for 30 min (cat. no. P0260; Beyotime Institute of Biotechnology), the other slide was used for IHC staining using mouse anti-human MMP-2 and mouse anti-human EphA2 (Boster cat. no. M00286 and BM0833, both 1:200) monoclonal antibodies detected by streptavidin-peroxidase or 3′-diaminobenzidine visualization kits (OriGene Technologies, Inc.).

Techniques: Expressing

Journal: Oncology Letters

Article Title: The combined use of EphA2/MMP-2 expression and MRI findings contributes to the determination of cerebral glioma grade

doi: 10.3892/ol.2019.10912

Figure Lengend Snippet: Immunohistochemical staining of glioma tissues. (A) MMP-2 and (B) EphA2 expression was identified by immunofluorescence staining in the cytoplasm of glioma cells. Positive cells were stained brown. Percentages of stained cells are presented in the bar graphs. Data represent the mean ± standard deviation (n=5). ***P<0.001. Scale bar, 30 µm. EphA2, ephrin type-A receptor 2; MMP-2, metalloproteinase 2.

Article Snippet: One slide was routinely stained with hematoxylin and eosin in 25°C for 1–5 min. After blocking in BSA solution 37°C for 30 min (cat. no. P0260; Beyotime Institute of Biotechnology), the other slide was used for IHC staining using mouse anti-human MMP-2 and mouse anti-human EphA2 (Boster cat. no. M00286 and BM0833, both 1:200) monoclonal antibodies detected by streptavidin-peroxidase or 3′-diaminobenzidine visualization kits (OriGene Technologies, Inc.).

Techniques: Immunohistochemical staining, Staining, Expressing, Immunofluorescence, Standard Deviation

Journal: Oncology Letters

Article Title: The combined use of EphA2/MMP-2 expression and MRI findings contributes to the determination of cerebral glioma grade

doi: 10.3892/ol.2019.10912

Figure Lengend Snippet: Pearson's correlation analysis of the magnetic resonance imaging parameters and immunohistochemistry results. Scatterplots presenting the correlation between (A) MMP-2 and (B) EphA2 positivity with the EI; (C) MMP-2 and (D) EphA2 positivity with the EP; and (E) MMP-2 and (F) EphA2 positivity with maximum tumor diameter. EI, edema index; EP, enhancement percentage; EphA2, ephrin type-A receptor 2; MMP-2, metalloproteinase 2.

Article Snippet: One slide was routinely stained with hematoxylin and eosin in 25°C for 1–5 min. After blocking in BSA solution 37°C for 30 min (cat. no. P0260; Beyotime Institute of Biotechnology), the other slide was used for IHC staining using mouse anti-human MMP-2 and mouse anti-human EphA2 (Boster cat. no. M00286 and BM0833, both 1:200) monoclonal antibodies detected by streptavidin-peroxidase or 3′-diaminobenzidine visualization kits (OriGene Technologies, Inc.).

Techniques: Magnetic Resonance Imaging, Immunohistochemistry

Journal: Frontiers in Cellular Neuroscience

Article Title: Gene expression profiling for human iPS-derived motor neurons from sporadic ALS patients reveals a strong association between mitochondrial functions and neurodegeneration

doi: 10.3389/fncel.2015.00289

Figure Lengend Snippet: Antibodies for characterization of hiPSC, embryoid body, differentiated motor neurons and cardiomyocytes .

Article Snippet: EphA2 , Rabbit IgG , Santa Cruz Biotechnology , sc-924 , 1:100.

Techniques: In Vitro

Journal: Frontiers in Cellular Neuroscience

Article Title: Gene expression profiling for human iPS-derived motor neurons from sporadic ALS patients reveals a strong association between mitochondrial functions and neurodegeneration

doi: 10.3389/fncel.2015.00289

Figure Lengend Snippet: Spontaneous differentiation of hiPSC into three germ layers in vitro and in vivo . Immunocytochemical staining of anti-Pan cytokeratin ( A ; Pck-26, a marker of ectoderm layer), Myosin Light Chain (LC) 2 ( B ; a marker of mesoderm layer) and Ephrin A2 ( C ; a marker of endoderm layer) after spontaneous differentiation in vitro of human-induced pluripotent stem cells (hiPSC). hiPSC were derived from fibroblasts which were obtained from motor nerve of sporadic ALS patient. Cell nuclei were stained with DAPI (blue; A–C ). Hematoxylin and eosin staining of sections from a teratoma formed after a subcutaneous injection of hiPSC into nude rats ( D–F ). Neural rosettes (arrow, D ), muscle tissue (arrow; E ), and gut-like epithelium (arrow, F ) indicate tissues into the teratomas with ectoderm, mesoderm and endoderm features, respectively. Scale bars: 50 μm.

Article Snippet: EphA2 , Rabbit IgG , Santa Cruz Biotechnology , sc-924 , 1:100.

Techniques: In Vitro, In Vivo, Staining, Marker, Derivative Assay, Injection